FIELD : medicine. SUBSTANCE : group of inventions relates to medicine, specifically to cardiovascular surgery. In manufacture of seal-containing conduit, its tubular body is obtained by cutting off from chemically stabilized venous vessel a fragment containing a seal with at least two cusps. A fragment of venous vessel, that is, the tubular body, is turned onto the side of the endothelial surface, seal cusps are checked and the first dilator is introduced into its lumen. After the introduction of the first dilator, pairs of marks are formed at the base of seals and at the tops of the commissures. Marks are formed by underruning of the wall of a fragment of venous vessel, that is, the wall of the tubular body, U-shaped raphes with atraumatic sutures, which lead to the outer surface of the fragment, turned out after removing the first dilator, with the endothelium inside. Check the location of marks and injected into the lumen of the fragment of the second dilator of larger diameter. Folds are formed on the wall of the fragment by a pairwise connection of atraumatic sutures, and then supporting elements are formed around the entire circumference of the fragment. Supporting elements are formed by imposing from the side of adventitia of a double-row continuous blanket surgical suture with the capture of all layers of the wall and folds, located at the base of the valves, and the imposition from the side of adventitia of a double-row continuous blanket surgical suture with pinch of all layers of the wall and folds located at the tops of commissures. By means of above mentioned method, seal-containing conduit is formed. EFFECT : group of inventions allows to ensure adequate blood flow in seal-containing conduit in pressure ranges, developed by ventricles of heart, reduce risk of dilatation of the seal area and significant regurgitation on the seal while maintaining elasticity of conduit walls for ease of positioning and implantation. 6 cl, 1 ex, 3 dwg