Provided in the present invention are an anti-PSCA and anti-PDL1 dual targeting chimeric antigen receptor based on the OCTS-CAR, an encoding gene, an OCTS-CAR-T recombinant expression vector and a construction method and use thereof. The anti-PSCA and anti-PDL1 dual targeting chimeric antigen receptor comprises a CD8 leader membrane receptor signal peptide, a dual antigen binding region, a CD8 Hinge chimeric receptor hinge, a CD8 Transmembrane chimeric receptor transmembrane region, a CD28 chimeric receptor costimulatory factor, an OX40 chimeric receptor costimulatory factor and a TCR chimeric receptor T-cell activation domain sequentially connected in series, wherein the dual antigen binding region comprises a heavy chain VH and a light chain VL of the PSCA and PDL1 single chain antibodies connected in series or in a turn connection manner, the hinge Inner-Linker within the antibody, and the hinge Inter-Linker between the single chain antibodies. In addition, also provided are the gene encoding the anti-PSCA and anti-PDL1 dual targeting chimeric antigen receptor, the recombinant expression vector and the construction method and use thereof.