Provided in the present invention are an OCTS-CAR dual targeting chimeric antigen receptor based on OCTS technology, an encoding gene, an OCTS-CAR-T recombinant expression vector and a construction method and the use thereof. The OCTS-CAR dual targeting chimeric antigen receptor comprises a CD8 leader membrane receptor signal peptide, a dual antigen binding region, a CD8 Hinge chimeric receptor hinge, a CD8 Transmembrane chimeric receptor transmembrane region, a CD28 chimeric receptor costimulatory factor, a CD134 chimeric receptor costimulatory factor and a TCR chimeric receptor T cell activation domain sequentially connected in series, wherein the dual antigen binding region comprises a heavy chain VH and a light chain VL of the two single chain antibodies connected in a certain manner, the hinge Inner-Linker within the antibody and the hinge Inter-Linker between the single chain antibodies, and the two single chain antibodies refer to the combination of any two of the BCMA single chain antibody, CD319 single chain antibody, CD38 single chain antibody, PDL1 single chain antibody and CD123 single chain antibody. In addition, also provided are the gene encoding the OCTS-CAR dual targeting chimeric antigen receptor, the recombinant expression vector and the construction method and use thereof.