The present invention relates to an eco-friendly manufacturing method for peptide derived from a cocoon and peptide derived from a cocoon manufactured by the same and, specifically, to a manufacturing method for peptide derived from a cocoon, applied with a non-chemical eco-friendly technique, and peptide derived from a cocoon, manufactured by the same. According to the present invention, the manufacturing method for peptide derived from a cocoon includes : (a) a step of cutting the cocoon; (b) a step of adding water to the cocoon which is cut and also performing sterilization and fluidization; (c) a step of high-shear mixing the cocoon fluidized; (d) a step of homogenizing the cocoon high-shear mixed by a high pressure homogenizer; (e) a step of forming the nano cocoon homogenized by a bead mill; and (f) a step of biologically processing the nano cocoon. According to the present invention, the manufacturing method for peptide derived from the cocoon has advantages such as reduced manufacturing processes, high productivity, reduced production costs, and eco-friendly manufacture by using eco-friendly decomposition method (physical cutting, top-down, and biological processing) which is not an existing chemical decomposition method. Also, the peptide derived from the cocoon manufactured by the same can be used as various materials such as a 3D printing material, cosmetics, a medical biological material, and functional health food by easily controlling the molecular weight of a final product.(AA) Microorganism incubation(B1, B2) Separation and purification process(CC) Concentration and enzyme production(DD) Cocoon(EE) Cutting process(FF) Fine cutting(GG) Sterilization and dissociation process(HH) Cutting and fluidization(II) Micro process(JJ) Homogenization process(KK) Nano process(LL) +commercial enzyme(MM) Enzyme treating process(NN) Concentration and drying process(OO) Cocoon pretreatment process(PP) Cocoon sterilization and fluidization process(QQ) Cocoon nano process(RR) Enzyme decomposition process(SS) Separation, purification, and drying processCOPYRIGHT KIPO 2019