The present invention relates to a process for obtaining compositions constituted by propolis nanoparticles and to the use thereof, said nanoparticles being optionally associated to one or more substances of interest such as active ingredients, as well as, optionally, substances of secondary effect such as synergists and adjuvants. The process for obtaining the nanoparticles comprises the steps of preparing a fraction A, which consists of propolis extract dissolved in an organic solvent, to which one or m ore stabilizers and/or emulsifiers may be added, as well as, optionally, one or more substances of interest and/or of secondary effect; ii) preparing a fraction B, aqueous phase, constituted by : (ii.1) water; or (ii.2) an aqueous solution or dispersion, to which one or more stabilizers and/or emulsifiers may be added; (iii) drooping the fraction A onto the fraction B or the fraction B onto the fraction A; iv) homogenizing the mixture by mechanical stirring and spontaneous formation of nanoaprticles with average size ranging from 1 to 1000 nm in a dispersion; v) optionally the following additional steps may be involved : (v-1) removing the organic solvent/ and/or (v-2) drying the nanodispersion. The developed compositions have applications in fields such as agrochemical, pharmaceutical, cosmetic or chemical fields.