The present method of purification of albumin provides within the sector of the chemistry and medicine, human albumin is for use in hospitals and clinics throughout the country, is to annotate the bovine albumin can be purified by the same method is used in all the world research laboratory. The method for the purification of albumin characterized in that it comprises the following steps : to) between 0 癱 to freeze -100 human plasma or bovine beta) thawing the plasma until reaching a temperature of between 0 C. to 38 癱) centrifuging plasma at 5000 - 15000 R p.m during 15 minutes to 8 c) discarding the supernatant precipitate and selecting and) adjust pH to 7 to 9 F. supernatant) adding ethanol to the supernatant until a 15 to 25% ratio in the supernatant to g)/ethanol solution to stand at least 6 hours supernatant to temperature not higher than 0 癱 to a H) centrifuging solution to cool 5000 - 15000 R p.m during 15 minutes 1 to 8) discard supernatant precipitate and selecting the anticipated) adjust pH to 6.9 4 supernatant as K) adding ethanol again until reaching a proportion between 12 to 24% of ethanol in the supernatant-L)/ethanol solution to stand at least 6 hours supernatant to temperature not higher than 0 癱 to mum) centrifuging solution to cool 5000 - 15000 R p.m during 15 minutes to 8 n 1) discarding the supernatant or precipitate and selecting) purified by exclusion chromatography circumvention the supernatant from the foregoing step P3) heating the supernatant of albumin purified from the previous step between 30 to 60 癱 by 1 to 4 hours I Q) cool supernatant of albumin from the foregoing step between 0 to 10 癱 for R) solution to cool centrifuging 5000 - 15000 R p.m during 15 minutes to 8 sec.) discard supernatant precipitate and selecting.