FIELD : medicine.SUBSTANCE : invention relates to medicine, in particular to immunology, and can be used to determine the content of anti-D antibodies in human immunoglobulin preparations in the hemagglutination reaction. For this, the kit contains two components that are solutions of human immunoglobulins : a positive component with a certified anti-D antibody content in the range from 1 : 4 to 1 : 8, anti-rhesus activity of not more than 0.7?IU/ml and certified protein content of not less than 25?mg/ml, obtained from blood plasma Rh(-) of donors of IV(AB) blood group; a negative component with a certified content of anti-D antibodies in an amount corresponding to a dilution of less than 1 : 2, undetectable anti-rhesus activity and a certified protein content of at least 25?mg/ml, obtained from blood plasma Rh(+) of donors of IV(AB) blood group.EFFECT : use of this invention allows to evaluate the quality of human immunoglobulin preparations and allows to determine the content of anti-D antibodies by hemagglutination.1 cl, 17 ex, 4 tbl