Disclosed are a 4-aminopyridine derivative, a pharmaceutical composition thereof, a preparation method therefor and a use thereof. The 4-aminopyridine derivative (I) of the present invention, and an isomer, prodrug, stable isotope derivative or pharmaceutically acceptable salt thereof have the following structure. The 4-aminopyridine derivative of the present invention has a good adenosine A2a receptor antagonistic action, and can effectively treat or relieve related diseases caused by an abnormal adenosine A2a receptor level, such as cancers, central nervous system diseases, etc. (I)