The present invention relates to a method for mass production of antifreeze proteins derived from mealworms and a technique related to antifreeze proteins thereby. According to the method for mass production of antifreeze proteins derived from mealworms of the present invention, by establishing a breeding condition for mass-expressing antifreeze proteins of mealworms, larvae of Tenebrio molitor, and a process for effectively separating and purifying antifreeze proteins in proteins of mealworms, it is possible to mass-produce antifreeze proteins derived from mealworms. Also, antifreeze proteins, produced by the mass production method of the antifreeze proteins derived from mealworms of the present invention, effectively lower the freezing point even at very low concentrations through direct interaction with ice, and thus can be effectively used as a material in various fields such as food, medical care, cosmetics, and agriculture.(AA) Mix 50g/100mL 50% ethanol(BB) Grind at a high speed, 5 minutes, 4 ° C(CC) Centrifugate 2100g, 30 minutes, -4 ° C(DD) Filter (pore size 11μ m)(E1, E2, E3) Dialyze (3.5K MWCO Dialysis tubing)(F1, F2, F3) Freeze-dryCOPYRIGHT KIPO 2019