Diabetes mellitus (DM) has turned out to be one of the major and emerging public health problems of the world. There has been perpetuating increase in the number of diabetic patients almost in all countries, especially in India which disreputably got nicknamed asthe Diabetes Capital of the World (Mohan et al., 2007). Diabetes mellitus is a complex and a diverse group of disorders that disturbs the metabolism of carbohydrate, fat and protein. Antioxidants are the compounds that can delay or inhibit the oxidation of lipids or the molecules by inhibiting the initiation or propagation of oxidative chain reactions. (Choe and Min, 2009), redox active compounds which help to prevent radical formation or remove them before damage can occur or repair oxidative damage. They eliminate damaged molecules by directly scavenging or neutralizing free radicals or other reactive oxygen species (ROS) and reactive nitrogen species (RNS). Human use of plants as medicinal purpose predates recorded history. Medicinal plant use data in many forms has been heavily utilized in the development of pharmacopoeias and formularies, providing a major focus in global healthcare, as well as contributing substantially to the drug development process for alternate of synthetic drug. Since ancient times, plants have been an exemplary source of medicine and many of the currently available drugs have been derived directly or indirectly from them. Ayurveda and other Indian literature mention the use of plants in treatment of various human ailments. The ethnobotanical information reports about 800 plants that may possess anti-diabetic potential. In present innovation, deals with the development of new formulation having potential anti-diabetic and anti-oxidant potential. In overall study following plants were investigated. 1) Artiplex hortensisL. 2) Chenopodium album L. 3) Senna glauca Roxb. These investigations are supported by determination of total flavonoid content, determination of total phenol content, DPPH free radical scavenging activity, ABTS radical scavenging activity, determination of a- Amylase inhibitory activity, determination of a-glucosidase inhibitory activity, determination of IC50 values. As results, the quantitative estimation of total phenols and flavonoids suggests that the S. glauca leaves are found to be rich source of phenol ics and flavonoids and its chloroform and ethanol extracts showed excellent DPPH and ABTS reduction. Based on IC50 values, it was revealed that the chloroform and ethanol extracts of both C. album and A. hortesnsis showed maximum radical scavenging activity. Overall, C album and A. hortesnsis would be a good source of antioxidants providing health-promoting effects in human. The study indicated that, the ethanolic extract of A. hortesnsis showed the maximum alpha amylase and alpha glucosidase inhibitory activity. Furthermore, combination of ethanolic extract of leaves of selected three plants in the formof new combination formulation viz. CAAH11, showed enhanced alpha amylase and alpha glucosidase inhibitory activity.