The purpose of the present invention is to provide a polypeptide having lipase activity with improved stability (heat, pH, solvent). The polypeptide at least includes, from the N-terminal side, β sheet (A), α helix (A), α helix (B), α helix (C), β sheet (B), and α helix (D), and has a lipase activity. The region between the β sheet (A) and the α helix (A) includes any of the amino acid residues shown in No. 1 - 125 of table I, and/or the region between the β sheet (B) and the α helix (D) includes any of the amino acid residues shown in No. 1 - 150 of table II.