The dihydropyrimidone was synthesized by Chemist Biginelli by using condensation reaction of 1, 3 dicarbonyl compound, benzaldehyde and urea this result in to pyrimidone derivative similarly pyrimidonethione have been synthesized by this method but thiourea is used in place of urea. The thiopyrimidone have numerous pharmaceutical applications and act as anti-ulcer, anti- inflammatory, anti-diabetic and anti-leishmanial drug. On other hand thiopyrimidone derivatives shows broad spectrum against pathogenic microorganisms and function as Anti-bacterial, antifungal, anti-viral and anti-metabolic activities. The thiopyrimidone have cytotoxic effect and also act as chemotherapeutic agent and have remarkable antitumor activities used as anti-cancer medicine. The pyrimidine thione derivatives found to possess anti-HIV activities. Such diverse application of pyrimidinthione derivatives in the field of medical and pharmaceutical science researcher attracted towards the synthesis of pyrimidinthione derivatives thus dipyrimidine-dithione derivative including cyclic imides moiety have synthesized starting from N-phenyl substituted cyclic imides to bis-heterocyclic chalcone which further allowed reacting with thiourea and finally furnished into Pyrrolo-dipyrimidine-dithione derivatives. The main objective of this invention is to synthesize the novel chemical compounds 4, 5-bis(N-phenyl)-9-(p-tolyl)- 3, 4, 4a, 4b, 5, 6-hexahydro-2H-pyrrolo[2, 3-d : 5, 4-d]dipyrimidine-2, 7(9H)-dithione and 9-(N-phenyl)-4, 5-bis(N-phenyl)- 3, 4, 4a, 4b, 5, 6-hexahydro-2H-pyrrolo[2, 3-d : 5, 4-d]dipyrimidine-2, 7(9H)-dithione series by using microwave assisted method and confirmed their antimicrobial activities. In this background the chemical moiety has secured better consideration as a potent assessment for good and specific route. In step of this important discussion, the synthesis of some novel bis-heterocylic chalcones based Pyrrolo-dipyrimidine-dithione derivatives gives significant anti-bacterial and anti-fungal activities.