FIELD : medicine.SUBSTANCE : invention refers to medicine, particularly phthisiology, and aims at detecting isolates of Mycobacterium tuberculosis Beijing 94-32-cluster. Availability of nucleotide substitution G>A in sigE gene in position 294 by means of PCR in real time format using oligonucleotide primers and fluorescent-labeled probes. PCR results are evaluated by recording a fluorescence signal on a HEX channel at wavelength of 555 nm, and with exponential accumulation it is determined whether M. tuberculosis belongs to Beijing 94-32-cluster. When detecting the fluorescence signal on the control FAM-channel with wave length 510 nm, the strain belongs to another genotype of M. tuberculosis.EFFECT : invention provides fast and reliable detection of tuberculosis mycobacteria Beijing 94-32-cluster genotype.1 cl, 3 dwg, 3 ex