The present invention falls within the field of biomedicine, and mainly provides a method for preparing and producing an antigen binding protein with regulated glycosylation modification, wherein the method comprises adding amino acids to the culture system during the cell culture cycle. The method can be used to regulate the glycosylation modification for the antigen binding protein, to improve the stability of the protein, to make the function associated with the complement-dependent cytotoxicity effect of the protein change, and to improve the ability to control the production process and the batch-to-batch consistency of the protein.