专利名称 | METHOD OF FORMATION OF THREE-DIMENSIONAL BIORESORBABLE STRUCTURES | ||
申请号 | RU2017147028 | 申请日 | |
公开(公告)号 | RU2691752C1 | 公开(公告)日 | |
申请(专利权)人 | Federalnoe gosudarstvennoe byudzhetnoe obrazovatelnoe uchrezhdenie vysshego obrazovaniya "Moskovskij gosudarstvennyj universitet imeni M V Lomonosova" (MGU) (RU) | 发明人 | Arkhipova Anastasiya Yurevna; Ramonova Alla Alikovna; Mojsenovich Mikhail Mikhajlovich; Bessonov Ivan Viktorovich; Kopitsyna Mariya Nikolaevna; Mashkov Aleksandr Evgenevich; Fedulov Aleksandr Vladimirovich; Soldatenko Anna Sergeevna; Shajtan Konstantin Voldemarovich |
专利来源 | 国家知识产权局 | 转化方式 | |
摘要 |
FIELD : medicine.SUBSTANCE : invention relates to medicine and biotechnology. Method for obtaining bioresorbable three-dimensional porous structures based on methacrylized gelatin and silk fibroin, comprising the following stages : dissolving silk fibroin in an aqueous solution of lithium bromide; freezing of an aqueous solution of silk fibroin and lyophilization; dissolving gelatin in potassium phosphate buffer and adding to the solution an excess of methacrylic anhydride; carrying out the reaction of methacrylization of gelatin; adding to the reaction mixture potassium phosphate buffer, followed by cooling and dialysis; freezing the resulting product and lyophilization; obtaining an aqueous solution of regenerated silk fibroin; obtaining a solution of methacrylated gelatin in DMSO and its cooling; adding a photoinitiator to the methacrylized gelatin solution; introducing a solution of regenerated silk fibroin into a mixture of methacrylized gelatin and a photoinitiator, followed by photopolymerization; treatment with 96?% ethyl alcohol hydrogels obtained after photopolymerization; removal of residual solvents.EFFECT : invention provides a high photopolymerization rate, as well as the possibility of obtaining porous structures of a given shape and with a controlled structure.4 cl, 2 ex, 1 dwg |
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