专利名称 | Recombinant Engineered Bacterium Co-Expressing Trans-Anethole Oxygenase and Formate Dehydrogenase and Application thereof in Production of Piperonal | ||
申请号 | US16517792 | 申请日 | |
公开(公告)号 | US20200010864A1 | 公开(公告)日 | |
申请(专利权)人 | Jiangnan University | 发明人 | Pu Zheng; Dan Wu; Peng Wen; Pengcheng Chen; Yin Fu |
专利来源 | 国家知识产权局 | 转化方式 | |
摘要 |
The present application discloses a method for producing piperonal by using a recombinant engineered bacterium co-expressing trans-anethole oxygenase and formate dehydrogenase, and an engineered bacterium thereof, including constructing a formate dehydrogenase gene fdh and trans-anethole oxygenase gene tao or trans-anethole oxygenase mutant gene co-expression recombinant vector; inductively expressing recombinant genetically engineered bacterium; and producing piperonal by using the recombinant genetically engineered bacterium. 15.91 g/L of piperonal with a transformation rate of 79.55% and a time-space transformation rate of 2.27 g/L/h can be finally obtained during catalysis, and the yield is significantly improved compared with the existing piperonal, thereby being more conducive to the smooth realization of industrial production. |
主管部门:海南中小企业服务 | 建设单位:海南商业联合会
版权所有:海南商业联合会 | 备案号:粤ICP备13083911号(ICP加挂服务)@2017