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专利概况
专利名称 Recombinant Engineered Bacterium Co-Expressing Trans-Anethole Oxygenase and Formate Dehydrogenase and Application thereof in Production of Piperonal
申请号 US16517792 申请日
公开(公告)号 US20200010864A1 公开(公告)日
申请(专利权)人 Jiangnan University 发明人 Pu Zheng; Dan Wu; Peng Wen; Pengcheng Chen; Yin Fu
专利来源 国家知识产权局 转化方式
摘要

The present application discloses a method for producing piperonal by using a recombinant engineered bacterium co-expressing trans-anethole oxygenase and formate dehydrogenase, and an engineered bacterium thereof, including constructing a formate dehydrogenase gene fdh and trans-anethole oxygenase gene tao or trans-anethole oxygenase mutant gene co-expression recombinant vector; inductively expressing recombinant genetically engineered bacterium; and producing piperonal by using the recombinant genetically engineered bacterium. 15.91 g/L of piperonal with a transformation rate of 79.55% and a time-space transformation rate of 2.27 g/L/h can be finally obtained during catalysis, and the yield is significantly improved compared with the existing piperonal, thereby being more conducive to the smooth realization of industrial production.

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