FIELD : medicine.SUBSTANCE : invention refers to medicine, namely to technology of freezing peripheral blood precursor cells with Hemicolite cryopreservation. Freezing of peripheral blood precursor cells (PBPC) is carried out in stages : mixing it with concentrate of PBPC in equal proportions in a disposable plastic container, then sealing it, passportization, placing in metal pencil-holder, followed by stage-by-stage freezing of cell suspension from plus 4?癈 to minus 80?癈 in electric refrigerators with adjustable cold adaptation temperature of cells, defrosting in water bath to temperature of plus 2??癈 in the cell suspension, wherein at the first stage of freezing, the containers with the PBPC suspension are held for 10 minutes in an electric freezer, with a set temperature of plus 4?癈, at the second stage, the biological object is transferred into an electric freezer at a temperature of minus 28??癈 is maintained in these conditions for 25 minutes, after which biological object is transferred into a freezer-storage with temperature minus 80??癈 for final freezing and prolonged cryopreservation of PBPC.EFFECT : invention improves the technological level and effectiveness of hematopoietic stem cell cryopreservation by using modern industrial equipment and minimizing the risks of post-transfusion complications, accessible to medical institutions experiencing problems in providing liquid nitrogen, and safe for health of technical personnel.1 cl, 1 tbl, 1 ex