The present invention discloses sporoderm-removed ganoderma lucidum spore powder, granule and a preparation method thereof. The sporoderm-removed ganoderma lucidum spore powder and granule is obtained from sporoderm-disrupted ganoderma lucidum spore powder through extraction, centrifugation, concentration, drying, and pulverization; and in the sporoderm-removed ganoderma lucidum spore powder and granule, the content of crude polysaccharides is 10-20 g/100 g, and the content of total triterpenes is 4-10 g/100 g. The present invention has beneficial effects of enhancing immunity and having an auxiliary protection function against radiation hazards. The content of active components is increased by processing the sporoderm-disrupted ganoderma lucidum spore powder through a special process, such that the human body can more effectively absorb the nutritional components contained in the ganoderma lucidum spores, and thus the ganoderma lucidum spore powder gives play to its efficacy better while it is convenient to carry and take the ganoderma lucidum spore powder; and both the sporoderm-removed ganoderma lucidum spore powder and granule of the present invention have inhibitory effects on the zebrafish human gastric cancer transplanted tumor, the zebrafish human lung adenocarcinoma transplanted tumor and the zebrafish human lymphatic cancer transplanted tumor, where the inhibitory effects on the zebrafish human gastric cancer transplanted tumor and zebrafish human lymphatic cancer transplanted tumor are stronger.