D2 Is a process for preparing a novel resolvin, analog using two kinds of lipoxygenase and a composition thereof, according to the present invention, Musse MoSus (. Mus musculus) Mice derived from 8- mice and tiandenia tiandensis (Burkholderia thailandensisOf) lipoxygenase from 15- strain in, environment-friendly way and not to date reported to date. The novel resolvin analog can be produced with high productivity and high yield, thereby being useful in various industrial fields such as, medicine, foods and cosmetics.