The present invention provides a method for extracting and isolating a lutein crystal from a vegetable oil resin containing a lutein diester, comprising the following steps : a) dissolving lipase into deionized water to form an enzyme solution; b) dissolving a lutein extract into an alcohol solvent containing the deionized water to form a uniform alcohol solution; c) adding the enzyme solution to the alcohol solution for performing hydrolysis, and stirring same to obtain a lutein solution; d) filtering and performing filtration isolation on the lutein solution to obtain a crystalline; e) re-dissolving the crystalline into a non-polar organic solvent, and using the deionized water for washing a water-soluble impurity; f) recycling the organic solvent and cooling same to obtain a recrystallization; and g) isolating the recrystallization and drying same to obtain the lutein crystal. According to the present invention, the selectivity is strong, the reaction time is short, no waste water is produced, the process is environment-friendly, the obtained lutein crystal is high in purity and yield, and the method is suitable for industrial production.