Use of berbamine dihydrochloride in preparation of an Ebola virus inhibitor. An antiviral active compound having binding ability to EBOV-GPc1 is obtained by using an activated Ebola virus envelope glycoprotein (EBOV-GPcl) as a target and performing structure-based virtual screening, said compound being berbamine dihydrochloride. The berbamine dihydrochloride can achieve the effect of anti-Ebola virus infection by binding to the target protein EBOV-GPcl so as to specifically inhibit the entry of Ebola recombinant virus. The concentration for 50% of maximum effect (EC50) of berbamine hydrochloride against EBOV is 0.49 μM, indicating that berberamine dihydrochloride has a strong inhibitory effect on EBOV.