Use of albiflorin or a pharmaceutically acceptable salt thereof in the preparation of a product for regulating and improving the balance of human intestinal flora. Albiflorin or a pharmaceutically acceptable salt thereof, as a regulator for improving the function of intestinal flora system, can be used in the preparation of a medicament, a health care product, a food, a food additive, or a nutritional supplement that ameliorates and treats a sub-health status and/or a disease caused by intestinal flora disturbance; the sub-health status is selected from intestinal system dysfunction and/or affective disorder; and the disease is selected from one or more of irritable bowel syndrome, depression, anxiety, colitis, and colon cancer.